ezrA [2019-07-10 13:54:41]

negative regulator of [protein|search|FtsZ ]ring formation

locus: BSU29610

pI: 4.76

mw: 64.82 kDa

protein length: 562 aa

gene length: 1689 bp

function: control of [protein|search|FtsZ ]ring formation

product: [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ]-interacting protein

essential: no

synonyms: ytwP

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,029,729 → 3,031,417

Phenotypes of a mutant

• altered localization of [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] and [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PBP1]]] [Pubmed|25403286]
• growth defects, elongated cells [Pubmed|25403286]
• the ''[gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]'' mutation is synthetically lethal with a ''[gene|DB09F1C36257F511A84A083967A25A9D46744D14|sepF]'' mutation [Pubmed|24218584]
• a ''[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]'' double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
• a ''[gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA] [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]'' double mutant forms filamentous cells [Pubmed|16420366]
• depletion of ''[gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]'' and deletion of ''[gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]'' have a strong synthetic defect in [SW|cell division] [Pubmed|24825009]

The protein

Catalyzed reaction/ biological activity

• inhibits [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] polymerization [Pubmed|25403286]
• recruits [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PBP1]]] to the division septum [Pubmed|25403286]
• activates [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC] [Pubmed|25845974]

Protein family

• EzrA family (single member, according to UniProt)

Effectors of protein activity

• degradation of [protein|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|EzrA] involves [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|FtsH] [Pubmed|24222488]

Structure

• [PDB|4UXV] (the 60 kDa cytoplasmic domain) [Pubmed|25403286]

[SW|Localization]

• membrane associated [Pubmed|18763711]
• 1 transmembrane domain at the N-terminus [Pubmed|25403286]

Expression and Regulation

Operons

• genes: [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]
  description: [Pubmed|9387221]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15317798], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulation

  • constitutively expressed [Pubmed|23701187]

  additional information

  • degradation of [protein|search|EzrA] involves [protein|search|FtsH] [PubMed|24222488]

Biological materials

Mutant

• MGNA-A428 (ytwP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/428 NBRP B. subtilis, Japan]
• 1A805 ( ''ezrA''::''kan''), [Pubmed|15101985], available at [http://bgsc.org BGSC]
• 1A1279 (''ezrA''::''spec''), [Pubmed|10449747], available at [http://bgsc.org BGSC]
• 1A1280 (''ezrA-gfp''), [Pubmed|10449747], available at [http://bgsc.org BGSC]
• BKE29610 (Δ[gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGAGCCCCCTTGCTG, downstream forward: _UP4_TAGATAATCACGACCATGAA
• BKK29610 (Δ[gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGAGCCCCCTTGCTG, downstream forward: _UP4_TAGATAATCACGACCATGAA

References

Reviews

• 19680248,19884039,28357277

Original Publications

• 10449747,16420366,15317782,16549676,17662947,12368265,18363795,9387221,15317798,19429628,18763711,21401734,24222488,23701187,17718511,24218584,24097947,24825009,25068683,25403286,25845974,25954268